Insertion junction: LMJ.RY0402.178584_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.178584
Chromosome:chromosome 6
Location:7499935
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g300450 (1 of 14) IPR003593//IPR003959//IPR027417 - AAA+ ATPase domain // ATPase, AAA-type, core // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACAAACGCACTTGCATGCGCTGCATTCA
Internal bar code:ACACCATCAGGGGTGTTCTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:287
LEAP-Seq percent confirming:57.3206
LEAP-Seq n confirming:599
LEAP-Seq n nonconfirming:446
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCATAGATTGATGCGGTA
Suggested primer 2:CTTTCTTCCCAATCTGCTGC