| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178599 |
| Chromosome: | chromosome 12 |
| Location: | 7139807 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559050 | (1 of 1) PTHR24006//PTHR24006:SF22 - FAMILY NOT NAMED // BCDNA.LD22910 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACCGTACGTGTTGCCCGCTACTGCGG |
| Internal bar code: | GAGCGGTATGTGAGGGTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 680 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCAAACACACTTCACG |
| Suggested primer 2: | TGAGCGCTCTCTTCTTCCTC |