| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.178609 |
| Chromosome: | chromosome 11 |
| Location: | 3172624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479350 | (1 of 3) PTHR31563//PTHR31563:SF1 - FAMILY NOT NAMED // ION CHANNEL POLLUX-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCTATTTACGGTTTGCAGGTAAGGTAC |
| Internal bar code: | AGTTCCTAATGAGTTGATACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 99.0566 |
| LEAP-Seq n confirming: | 1365 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACATGCATTGCACCCTGTT |
| Suggested primer 2: | GCCAGCTGATCAAGAAGGAC |