| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178632 |
| Chromosome: | chromosome 17 |
| Location: | 1068613 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703750 | (1 of 1) PF14222 - Cell morphogenesis N-terminal (MOR2-PAG1_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGGTGTTGGGCCACCACCTGCTCAAC |
| Internal bar code: | TTTGACGTGCGTGACTACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 89.8636 |
| LEAP-Seq n confirming: | 5337 |
| LEAP-Seq n nonconfirming: | 602 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCATGCGAGGAAGACCT |
| Suggested primer 2: | AAATCCAAGTCCAAACGCAG |