Insertion junction: LMJ.RY0402.178647_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.178647
Chromosome:chromosome 3
Location:5484451
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g185500 VIP1,FAP160 Diphosphoinositol Pentakisphosphate Kinase; (1 of 2) 2.7.4.21//2.7.4.24 - Inositol-hexakisphosphate kinase / IP6K // Diphosphoinositol-pentakisphosphate kinase / PP-IP(5) kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTACTTCACGTCGGAGTCGCACATGCAC
Internal bar code:ATGAGGCGCTCACGTAGTAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:83.9196
LEAP-Seq n confirming:167
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACGAATGAGTTGCACTG
Suggested primer 2:GGGGAAAGAGGGTTGAAGAG