| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178665 |
| Chromosome: | chromosome 6 |
| Location: | 4329282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278261 | (1 of 1) PTHR13774:SF17 - PHENAZINE BIOSYNTHESIS-LIKE DOMAIN-CONTAINING PROTEIN | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGTGCCAAGGCCCCGAGTAGTCGGCAC |
| Internal bar code: | CGGAGCTGCGAAAAGCATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.8821 |
| LEAP-Seq n confirming: | 3388 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGTCTCGCACCGTTCCTC |
| Suggested primer 2: | TTGCACGTGAATAGGGAACA |