| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178680 |
| Chromosome: | chromosome 4 |
| Location: | 1343252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214600 | (1 of 30) 3.1.3.48 - Protein-tyrosine-phosphatase / PTPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAACTTAACACCGAAACGTATGGCCG |
| Internal bar code: | CAGACCTTCTGGAAGCCAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGGTCATCTACGCAT |
| Suggested primer 2: | AAATGCTTTGGTTGGTACGG |