| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178685 |
| Chromosome: | chromosome_8 |
| Location: | 3573008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g375700 | SMM31 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCGGCCTGCAATGCACTCCGGCCTAGCC |
| Internal bar code: | GGAACAGCATATCGTGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 98.3861 |
| LEAP-Seq n confirming: | 4633 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAAGCGGATCATAGCTG |
| Suggested primer 2: | GCTCTTCAAATATCGGAGCG |