| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178705 |
| Chromosome: | chromosome 14 |
| Location: | 1441916 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617800 | (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGCAGCTGGGCGTGCTGAGCAGCGGTA |
| Internal bar code: | ACAGCAAAAAGGGACGCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.7709 |
| LEAP-Seq n confirming: | 871 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCATTATGGGTGAGGGGA |
| Suggested primer 2: | ACCCAGTGTAATGGAGCAGG |