| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178770 |
| Chromosome: | chromosome 1 |
| Location: | 371087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002250 | NAT4 | (1 of 1) PTHR23091//PTHR23091:SF206 - N-TERMINAL ACETYLTRANSFERASE // ACYL-COA N-ACYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; Histone acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTCTCACCGTGCCTTCATTAAGCCGGT |
| Internal bar code: | ACGATCGTGCTTTCATATGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 99.7105 |
| LEAP-Seq n confirming: | 2411 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACACTCCCTACACCCA |
| Suggested primer 2: | TCGAGTATGCAAGGTGAACG |