| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178779 |
| Chromosome: | chromosome 2 |
| Location: | 7015431 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147700 | (1 of 1) PTHR11271//PTHR11271:SF33 - CHLOROHYDROLASE FAMILY PROTEINS // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGTTTGGAAGCGCATGTGCAGATCTCC |
| Internal bar code: | GTGCCCGTGCAACAGAGTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.7399 |
| LEAP-Seq n confirming: | 4601 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACGGACACCTACCACGC |
| Suggested primer 2: | GGGCTAAGACGTAGGGGTTC |