Insertion junction: LMJ.RY0402.178787_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.178787
Chromosome:chromosome 7
Location:1789295
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325723 ADK1 Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF85 - NUCLEOTIDE KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCGTCGGCGACAATCAAAAGAATACAT
Internal bar code:GAGCTCCTGTTGTTGGCACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1108
LEAP-Seq percent confirming:98.4227
LEAP-Seq n confirming:312
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGGCGCTGTATTTAGTC
Suggested primer 2:GTGTTTCTTTCTCCGCTTCG