| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178843 |
| Chromosome: | chromosome 1 |
| Location: | 5071220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035100 | (1 of 1) 5.4.99.18 - 5-(carboxyamino)imidazole ribonucleotide mutase / N(5)-carboxyaminoimidazole ribonucleotide mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCTGCACTCAACAAGTTGCTGCCGCGG |
| Internal bar code: | CTGCATGTCTATGCTAGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.6434 |
| LEAP-Seq n confirming: | 3633 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCTTGCTTGAGGTTGG |
| Suggested primer 2: | AAGATACGACCCCTGCACAC |