Insertion junction: LMJ.RY0402.178858_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.178858
Chromosome:chromosome 10
Location:2009010
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g432600 ARS18 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCAACCGCTCCAGTCGCGCTCATTGAC
Internal bar code:CTGTACCATGCGTGTGCGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1138
LEAP-Seq percent confirming:99.7602
LEAP-Seq n confirming:1248
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACACCGT
Suggested primer 2:AACGCAATCTTCCGTTCATC