| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178867 |
| Chromosome: | chromosome 9 |
| Location: | 2672314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389850 | (1 of 29) IPR013320 - Concanavalin A-like lectin/glucanase domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGCCAGCGTCCTGCACTTTCCAGCAA |
| Internal bar code: | GGGCACGACGGACTAGCGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.5918 |
| LEAP-Seq n confirming: | 1464 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTTGGGATGATGTAAGT |
| Suggested primer 2: | GAACCGAAGATACGCAGCTC |