| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178875 |
| Chromosome: | chromosome 16 |
| Location: | 1662294 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654550 | (1 of 5) 1.14.11.27 - [Histone H3]-lysine-36 demethylase / JmjC domain-containing histone demethylase 1A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGCGCACAAACCCCACAACCGGGTTAC |
| Internal bar code: | AGTGGTTTCCCGGCATTGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTGATGCATCTTTGAA |
| Suggested primer 2: | GGATTTGGGTCCACTTGAGA |