| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.178893 |
| Chromosome: | chromosome 1 |
| Location: | 5787855 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041351 | (1 of 1) PTHR15967:SF0 - E2F-ASSOCIATED PHOSPHOPROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCCTGCAGGTGCTGTTGTTGATGCTGA |
| Internal bar code: | AAGGGGTACGTAGGGTTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 98.6745 |
| LEAP-Seq n confirming: | 1340 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCAATCCCTCATACAGC |
| Suggested primer 2: | GTCCATGAACGTGACCACAG |