| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.178903 |
| Chromosome: | chromosome 3 |
| Location: | 5882061 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCACCCCCATTGCTATTCAGGGGGGAGC |
| Internal bar code: | TGCCGTGCTTATGCACTCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 98.3531 |
| LEAP-Seq n confirming: | 2568 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACCAGAAGCTGTTTCA |
| Suggested primer 2: | CAATGGCGCACTCTTACTCA |