| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.178944 |
| Chromosome: | chromosome 6 |
| Location: | 2929587 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272701 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCCTAGTCGACTCTTCCGCCCAAAAC |
| Internal bar code: | ATGAGCTCCGGAAAGTCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 88.6676 |
| LEAP-Seq n confirming: | 1870 |
| LEAP-Seq n nonconfirming: | 239 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTCAGCTTCACAAC |
| Suggested primer 2: | AATTTAGTGGGGCGACAGTG |