| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.178958 |
| Chromosome: | chromosome 3 |
| Location: | 4184789 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173900 | (1 of 5) IPR001841//IPR013083 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGGTGAATGATGACTGGGGTGGCGCA |
| Internal bar code: | ACTCGATCCTTCGGTCTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.517 |
| LEAP-Seq n confirming: | 14011 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAAACCAAAGAGGCTGC |
| Suggested primer 2: | ACCCCTCCTACACAGGCTCT |