Insertion junction: LMJ.RY0402.178986_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.178986
Chromosome:chromosome 6
Location:2696989
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g270400 GT90-6,GT90F6 GT90 family protein 6; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACTCGCGCTGTACACACTTGCCTCTGC
Internal bar code:CTCTGTTTGTGCGGTGAAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1009
LEAP-Seq percent confirming:87.1389
LEAP-Seq n confirming:1267
LEAP-Seq n nonconfirming:187
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TCACTGTCTCGCCAACTGAC
Suggested primer 2:AGGTCTGTCAGGTGGTGGTC