| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179014 |
| Chromosome: | chromosome 1 |
| Location: | 2958091 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018050 | NEF1 | (1 of 1) PTHR35313//PTHR35313:SF1 - FAMILY NOT NAMED // NO EXINE FORMATION 1; NEF1-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTAACGCGTACTCCCGTGCTGCTTCCC |
| Internal bar code: | ACCGCATGTGGTGTGGTCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 98.9933 |
| LEAP-Seq n confirming: | 590 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCCTTGCTTCTCTTTTA |
| Suggested primer 2: | GTACAGCAGCAGCACATGGT |