Insertion junction: LMJ.RY0402.179021_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179021
Chromosome:chromosome 15
Location:992640
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g639000 OPR88,RAP22,CLR11 RAP-domain containing protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACGCGACGCGGGCGTGAACATTCCCA
Internal bar code:AGGTGGCGCGTGGCTCGACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:506
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:268
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCAGCGCTCACATACCA
Suggested primer 2:TTCCCAGATCCCAGAAGATG