| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179030 |
| Chromosome: | chromosome 1 |
| Location: | 4562408 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031100 | MET1,TEF30 | Thylakoid enriched fraction, 30-kDa protein; (1 of 3) PTHR17130//PTHR17130:SF24 - MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25 // GAN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAAGCAATGAAGACAGCATGGATTCACG |
| Internal bar code: | CGTCAGCGGTACACATGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 99.8584 |
| LEAP-Seq n confirming: | 2115 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGCAATGCCTGAAGAGT |
| Suggested primer 2: | TCTCGTTAACCGGTTCCATC |