| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179039 |
| Chromosome: | chromosome 8 |
| Location: | 4155825 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379950 | PFH17,P4H17,PHX12 | Prolyl 4-hydroxylase 17; (1 of 2) PTHR10869//PTHR10869:SF79 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGACACACCTTTTGCGGACAAGTCATAC |
| Internal bar code: | CGGGGTCGGTACGGGTTACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 81.7144 |
| LEAP-Seq n confirming: | 4366 |
| LEAP-Seq n nonconfirming: | 977 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | CAGATCTCCTTTGAGCTGCC |