| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179095 |
| Chromosome: | chromosome 9 |
| Location: | 6130133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405200 | MRPL13,uL13m | Mitochondrial ribosomal protein L13; (1 of 2) K02871 - large subunit ribosomal protein L13 (RP-L13, MRPL13, rplM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGTCGGCGCCTGCAGCAGCAGCAGCA |
| Internal bar code: | TTGCTGAGCTTCTGTTGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1209 |
| LEAP-Seq percent confirming: | 97.9355 |
| LEAP-Seq n confirming: | 5645 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGCTCAGATCGCACAAC |
| Suggested primer 2: | CATTCCCATTGAACATTCCC |