| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179120 |
| Chromosome: | chromosome 1 |
| Location: | 5369374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037700 | FAD3,FAD9,delta4FAD | (1 of 2) PTHR19353//PTHR19353:SF30 - FATTY ACID DESATURASE 2 // FATTY ACID DESATURASE 2; Delta4-fatty acid desaturase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTATTGAGCATCTCTGTAGGCAAGTTT |
| Internal bar code: | AGGCGAGGAGCGGAGTCTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1042 |
| LEAP-Seq percent confirming: | 99.6088 |
| LEAP-Seq n confirming: | 1273 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTCCTGCTGTACGGCCT |
| Suggested primer 2: | GCTTGGCAAAAGCAAGAAAC |