| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179129 |
| Chromosome: | chromosome 14 |
| Location: | 1236098 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616350 | FAS4 | Fasciclin-like protein 4; (1 of 10) PTHR10900 - PERIOSTIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAAAACGCCACCATCCAGTTCCGTGTCT |
| Internal bar code: | GGGCCCACACACAACATAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 97.5845 |
| LEAP-Seq n confirming: | 2828 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTAGCGGAGCATAGGCA |
| Suggested primer 2: | CCAACTGGTAGCCCTGAGAG |