| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179134 |
| Chromosome: | chromosome 9 |
| Location: | 4066163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393395 | (1 of 1) PTHR33477:SF3 - EMB|CAB72147.1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATGGTATGAAGTTCGGGCCCTCGTGT |
| Internal bar code: | GTGGGAATGGTCCCTGTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 99.6416 |
| LEAP-Seq n confirming: | 278 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTGCATGAGTGAGAGG |
| Suggested primer 2: | AAGCAGTATCGGAGCTGGAA |