| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179155 |
| Chromosome: | chromosome 16 |
| Location: | 6318292 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674500 | ABCA4 | ABC transporter 4; (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCAGCCGGGGCTGCTGGTGGGGCAACT |
| Internal bar code: | GCCCGGTCAATCCGTTAATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 99.8248 |
| LEAP-Seq n confirming: | 4557 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGGACAGCAATGGAGG |
| Suggested primer 2: | TAGGTGTGTGGGGAGAGGAG |