| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179187 |
| Chromosome: | chromosome 13 |
| Location: | 4807085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605250 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTTGGCTTTGACCGTGTACCCTTTCAC |
| Internal bar code: | GGCCGTCATAACCCGGAATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 98.7594 |
| LEAP-Seq n confirming: | 2627 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGATATGCGTTTAACCT |
| Suggested primer 2: | AGTCCTCCATGCCATACTCG |