Insertion junction: LMJ.RY0402.179188_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179188
Chromosome:chromosome 12
Location:5873456
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g533800 CYN22 Cyclophilin 22; (1 of 1) K09567 - peptidyl-prolyl isomerase H (cyclophilin H) (PPIH, CYPH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCTCACCTTAACCTTCCTGTCGCAGTA
Internal bar code:GGTACTTCTCGGGCACTGGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:858
LEAP-Seq percent confirming:99.8086
LEAP-Seq n confirming:1043
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCAGAAAGCTTGCAGAC
Suggested primer 2:CCAGACCCCAACACTCAGTT