| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179192 |
| Chromosome: | chromosome 9 |
| Location: | 6452991 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTTTGTTTGGGCTGGTACTTACAACCC |
| Internal bar code: | GGGGGGTCCCCCAAATCAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 96.9724 |
| LEAP-Seq n confirming: | 5509 |
| LEAP-Seq n nonconfirming: | 172 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGGGGGTGACTCTAAA |
| Suggested primer 2: | CATGGGGTAGAAGGAGAGCA |