| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179192 |
| Chromosome: | chromosome 12 |
| Location: | 5554710 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531450 | HPAT3 | Hydroxyproline O-arabinosyl transferase 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGTGTCATGCTGCGGCGGCAGGGGCA |
| Internal bar code: | GTGTGGCCAATCATTGGTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1144 |
| LEAP-Seq percent confirming: | 99.5365 |
| LEAP-Seq n confirming: | 13958 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTATCACTACGTGGCGGG |
| Suggested primer 2: | CAGTCGGGAGCACTTGGTAT |