| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179211 |
| Chromosome: | chromosome 1 |
| Location: | 5627878 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040000 | RPL26 | (1 of 1) K02898 - large subunit ribosomal protein L26e (RP-L26e, RPL26); Cytosolic 80S ribosomal protein L26 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCGAGGGTTGCACGGGGGATGGATGG |
| Internal bar code: | AGACCCCGAGTCGGACGACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 41.5249 |
| LEAP-Seq n confirming: | 659 |
| LEAP-Seq n nonconfirming: | 928 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCGCAAGGTGTGTAGTG |
| Suggested primer 2: | CCAGTGTGCCTCCAACTTTT |