| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179222 |
| Chromosome: | chromosome 17 |
| Location: | 6084046 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCTCGAGTAGCCCTGCTGCCGGCATGGT |
| Internal bar code: | CTGTGAAGCCGGGCGCAGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 41.958 |
| LEAP-Seq n confirming: | 360 |
| LEAP-Seq n nonconfirming: | 498 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGAGGTATCGCACCAG |
| Suggested primer 2: | GGCCTTGACACATCACACAC |