Insertion junction: LMJ.RY0402.179229_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179229
Chromosome:chromosome 5
Location:2817570
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g237450 PSRP1 Plastid-specific ribosomal protein 1; (1 of 1) PTHR33231//PTHR33231:SF1 - FAMILY NOT NAMED // 30S RIBOSOMAL PROTEIN-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACATAGGCAGGCTGCGGTTCCTGTTTGCC
Internal bar code:GTGACCATAACGAACGGGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:361
LEAP-Seq percent confirming:91.1079
LEAP-Seq n confirming:625
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTCACCCTCACTTGCAC
Suggested primer 2:TCGAAATCTTGTGCAACAGC