| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179229 |
| Chromosome: | chromosome 5 |
| Location: | 2817588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237450 | PSRP1 | Plastid-specific ribosomal protein 1; (1 of 1) PTHR33231//PTHR33231:SF1 - FAMILY NOT NAMED // 30S RIBOSOMAL PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGTCCGCATGCAATCCGGCCTGGCTGC |
| Internal bar code: | TGGCAGCGGCCTATATGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.5449 |
| LEAP-Seq n confirming: | 2406 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTCACCCTCACTTGCAC |
| Suggested primer 2: | TCGAAATCTTGTGCAACAGC |