| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179232 |
| Chromosome: | chromosome 10 |
| Location: | 2931668 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440350 | (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACTCGGATGCCGGCGCCGCAGCGCGC |
| Internal bar code: | CCACGTCACGGGGAAGGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 97.4719 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTCCACGTCTGTCAAG |
| Suggested primer 2: | CTGAGCTAGCTTCCACCCAC |