| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179258 |
| Chromosome: | chromosome 1 |
| Location: | 7208130 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051800 | AAT,AST2 | (1 of 1) PTHR11751//PTHR11751:SF184 - SUBGROUP I AMINOTRANSFERASE RELATED // SUBFAMILY NOT NAMED; Aspartate aminotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATCTCCAGCACCATTGCTCGACTACAGC |
| Internal bar code: | GCGCCCATCAAGCCGACATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 99.759 |
| LEAP-Seq n confirming: | 1242 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGAACATCATAAGCGT |
| Suggested primer 2: | TTGACGTCTGCGCTTCATAC |