| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179283 |
| Chromosome: | chromosome 13 |
| Location: | 1406248 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571950 | (1 of 1) 2.1.1.104 - Caffeoyl-CoA O-methyltransferase / Trans-caffeoyl-CoA 3-O-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGAATGCACGACGCTTGGTGATTTGCAA |
| Internal bar code: | TGAAACGACGCAGTCAAGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 80.9004 |
| LEAP-Seq n confirming: | 6146 |
| LEAP-Seq n nonconfirming: | 1451 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGTCAGGCTAGGGACT |
| Suggested primer 2: | TATTGGTGGAGGGAGCAGAC |