| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179290 |
| Chromosome: | chromosome 10 |
| Location: | 3261566 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443150 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGGCTTGGACGTATGGGAGCGGGTTGG |
| Internal bar code: | AGATCCGTGTGACTGTCATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.9218 |
| LEAP-Seq n confirming: | 1278 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATCCGGTGTTCTTGACTT |
| Suggested primer 2: | AGTCCAGGACGTGCGAGTAG |