| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179295 |
| Chromosome: | chromosome 9 |
| Location: | 3352399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388615 | (1 of 1) K06965 - protein pelota (PELO, DOM34, pelA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCGTGGATGGCCACAACTTCGCTTTTT |
| Internal bar code: | ACATTGGTTTGCCACTGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.2857 |
| LEAP-Seq n confirming: | 1390 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACTCGTTGGAATTGGAG |
| Suggested primer 2: | GCTCCTTGAACTGGTCCTTG |