| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179315 |
| Chromosome: | chromosome_7 |
| Location: | 1371721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g322550 | FAP240 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGCCTGTACGTATAATACGGTCCGACTTA |
| Internal bar code: | GCTTACACGCGAGAACGAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 94.7034 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGATGAGTGTCACCGCTA |
| Suggested primer 2: | ACCTTCCAGACCAACGTGTC |