Insertion junction: LMJ.RY0402.179325_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179325
Chromosome:chromosome 8
Location:939091
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g361900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGAGTGGGTGGAGTGCTTCTTCGACGG
Internal bar code:ACGGGAGACGCGCGCCCGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1066
LEAP-Seq percent confirming:82.0248
LEAP-Seq n confirming:4764
LEAP-Seq n nonconfirming:1044
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAGTAAGGTGTGCAGGA
Suggested primer 2:AAAGGCTATGTGGTGATGCC