| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.179339 |
| Chromosome: | chromosome_6 |
| Location: | 2790943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g271150 | FAP74 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGACCTGGGGGGGATGAGGAAAAGCAGGG |
| Internal bar code: | ACTGTCGGGATAGGCGTGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 99.8692 |
| LEAP-Seq n confirming: | 1527 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCATGAAGGCAGTCACA |
| Suggested primer 2: | ACGTGAGTGTGTAGGGGGTC |