| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.179349 |
| Chromosome: | chromosome 1 |
| Location: | 6342179 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045902 | HCF101 | [4Fe-4S]-cluster scaffold protein; (1 of 1) PTHR23264//PTHR23264:SF29 - NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED // PROTEIN HIGH CHLOROPHYLL FLUORESCENCE 101 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGTGCGTGAAGGGTGTGGGGATAGA |
| Internal bar code: | CCTGCGACGCGGGGTCCCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 59.0 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTGCCCAGTGTTTGTT |
| Suggested primer 2: | CCGGTAACGATTGCTGTTCT |