| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.179354 |
| Chromosome: | chromosome 7 |
| Location: | 5643700 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352251 | (1 of 1) PTHR18934//PTHR18934:SF99 - ATP-DEPENDENT RNA HELICASE // ATP-DEPENDENT RNA HELICASE DHX37-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCCCCTCTCCCGCCCCCGCCCCAGCAC |
| Internal bar code: | CACAGTGAAATGCTTATGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 2.12766 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCCTCCTCCTTCACTGC |
| Suggested primer 2: | GTTTCGGATACGTGCAACCT |