| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.179358 |
| Chromosome: | chromosome 2 |
| Location: | 7166499 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146450 | CYG60 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAATTACCAATGCCACTTGGTTGGGCGC |
| Internal bar code: | GGCGTCAGGTGTGGACGTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 99.8891 |
| LEAP-Seq n confirming: | 901 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGCTGAAGAACATCCA |
| Suggested primer 2: | AGAGCAGTGCTGAGGTGGAT |