Insertion junction: LMJ.RY0402.179369_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.179369
Chromosome:chromosome 7
Location:1811047
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g325727 (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACATGAAGCTGCTCAGATGTACACGGGC
Internal bar code:GAGTAGAGGGGGAGCAGAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:604
LEAP-Seq percent confirming:99.9038
LEAP-Seq n confirming:2078
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTCTGCACGTGTGAGTG
Suggested primer 2:TGACATGGTTTGTTGCGTTT